Hematopoietic stem cell transplantation in its 60s: a platform for cellular therapies. From haematopoietic stem cells to complex differentiation landscapes. Hematopoietic stem cells: concepts, definitions, and the new reality. Therefore, this study uncovers the previously inaccessible kinetics and fate choices of transplanted HSCs in myeloablated recipients at early stage, with implications for clinical applications of HSCs and other stem cells.Įaves, C. Parallel in vitro and in vivo functional experiments supported the paradigm of robust differentiation without substantial HSC expansion during the first week. Based on our transcriptional classifications, most homed HSCs in bone marrow and spleen became multipotent progenitors and, occasionally, some HSCs gave rise to megakaryocytic–erythroid or myeloid precursors. We then applied them in conjunction with functional assays to track the dynamic changes of immunophenotypically purified HSCs in irradiated recipients within the first week after transplantation.
Here, using single-cell RNA sequencing, we first obtained the transcriptome-based classifications of 28 haematopoietic cell types. Improved menubar and Dock handling in full screen mode on Mac OS X 10.2 Jaguar or later.Fixed an issue with Mac OS X 10.5 Leopard that caused the game application to not exit full screen mode when displaying Help pages.Improved Preferences dialog appearance on Mac OS X 10.4 Tiger or later (also fixed tab pane drawing problems on 10.5 Leopard).Fixed broken functionality of Application menu items "Hide MacPipes X", "Hide Others" and "Show All".Updated the Kagi Registration Module (fixed issues with Mac OS X 10.5 Leopard).Other minor bug fixes.How transplanted haematopoietic stem cells (HSCs) behave soon after they reside in a preconditioned host has not been studied due to technical limitations.
Updated the Kagi Registration Module (fixed issues with Mac OS X 10.5 Leopard). Improved Preferences dialog appearance on Mac OS X 10.4 Tiger or later (also fixed tab pane drawing problems on 10.5 Leopard).įixed broken functionality of Application menu items "Hide MacPipes X", "Hide Others" and "Show All". Improved menubar and Dock handling in full screen mode on Mac OS X 10.2 Jaguar or later.įixed an issue with Mac OS X 10.5 Leopard that caused the game application to not exit full screen mode when displaying Help pages.